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Antibodies bound to the blocking peptide not bind to the epitope over the concentrate on protein. By comparing the staining from your blocked antibody versus the antibody alone, one can see which staining is unique.
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This manual outlines more achievable optimization of mobile and immunoassay parameters to be sure the best possible final results are...
This tutorial outlines even more feasible optimization of cellular and immunoassay parameters to be certain the very best effects are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind specially towards the target antibody and block antibody binding. These peptide usually is made up of the epitope recognized by the antibody.
Goal Information and facts Cyclin E belongs to the extremely conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which contribute to the temporal coordination of each mitotic function. Cyclin E sorts a complex with and features for a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and it is degraded as cells development via S period.
Overexpression of the gene continues to be noticed in many tumors, which leads to chromosome instability, and so may lead to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in selling cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two added splice variants were being documented but detailed nucleotide sequence facts just isn't yet readily available.
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